LOCUS       XM_009313852            2661 bp    mRNA    linear   INV 09-JUN-2015
DEFINITION  Trypanosoma grayi putative argonaute-like protein partial mRNA.
ACCESSION   XM_009313852
VERSION     XM_009313852.1
DBLINK      BioProject: PRJNA258390
            BioSample: SAMN02726834
KEYWORDS    RefSeq.
SOURCE      Trypanosoma grayi
  ORGANISM  Trypanosoma grayi
            Eukaryota; Discoba; Euglenozoa; Kinetoplastea; Metakinetoplastina;
            Trypanosomatida; Trypanosomatidae; Trypanosoma.
REFERENCE   1  (bases 1 to 2661)
  AUTHORS   Kelly,S., Ivens,A.C., Manna,P.T., Gibson,W. and Field,M.C.
  TITLE     A draft genome for the African crocodilian trypanosome Trypanosoma
            grayi
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2661)
  CONSRTM   NCBI Genome Project
  TITLE     Direct Submission
  JOURNAL   Submitted (09-JUN-2015) National Center for Biotechnology
            Information, NIH, Bethesda, MD 20894, USA
REFERENCE   3  (bases 1 to 2661)
  AUTHORS   Kelly,S., Ivens,A.C., Manna,P.T., Gibson,W. and Field,M.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-JUN-2014) University of Edinburgh, Kings Buildings,
            Mayfield Rd, Edinburgh EH9 3JT, United Kingdom
REFERENCE   4  (bases 1 to 2661)
  AUTHORS   Kelly,S., Ivens,A.C., Manna,P.T., Gibson,W. and Field,M.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAY-2014) University of Edinburgh, Kings Buildings,
            Mayfield Rd, Edinburgh EH9 3JT, United Kingdom
COMMENT     PROVISIONAL REFSEQ: This record has not yet been subject to final
            NCBI review. This record is derived from an annotated genomic
            sequence (NW_008826108).
            COMPLETENESS: incomplete on both ends.
FEATURES             Location/Qualifiers
     source          1..2661
                     /organism="Trypanosoma grayi"
                     /mol_type="mRNA"
                     /strain="ANR4"
                     /host="Crocodylus niloticus"
                     /db_xref="taxon:71804"
                     /geo_loc_name="Gambia"
                     /collection_date="1990"
                     /note="in vitro culture"
     gene            <1..>2661
                     /locus_tag="DQ04_04891000"
                     /db_xref="GeneID:20384129"
     CDS             1..2661
                     /locus_tag="DQ04_04891000"
                     /inference="protein motif:Gene3D:2.170.260.10"
                     /inference="protein motif:PANTHER:PTHR22892"
                     /inference="protein motif:SMART:SM00949"
                     /inference="protein motif:SMART:SM00950"
                     /inference="protein motif:SUPERFAMILY:SSF101690"
                     /inference="protein motif:SUPERFAMILY:SSF53098"
                     /note="automatic annotation, product description inferred
                     from BLASTP similarity;
                     predicted domains; G3DSA:2.170.260.10: paz
                     domain|aa:283-376|eval:4.3E-4; PTHR22892:
                     piwi-related|aa:4-886|eval:1.5E-149; PTHR22892:SF21:
                     NA|aa:4-886|eval:1.5E-149; PF02170: PAZ
                     domain|aa:276-389|eval:1.8E-7; PF02171: Piwi
                     domain|aa:543-864|eval:2.9E-14; SM00949:
                     |aa:274-404|eval:1.0E-10; SM00950:
                     |aa:539-871|eval:1.2E-22; SSF101690: PAZ
                     domain|aa:260-415|eval:3.92E-11; SSF53098: Ribonuclease
                     H-like|aa:537-667|eval:1.06E-24; SSF53098: Ribonuclease
                     H-like|aa:694-865|eval:1.06E-24;
                     50pc ID to unnamed protein product [Trypanosoma congolense
                     IL3000|protein GI:342184654], bitscore:872;
                     50pc ID to putative argonaute-like protein [Trypanosoma
                     congolense IL3000|protein GI:342184655], bitscore:869;
                     50pc ID to argonaute-like protein 1 [Trypanosoma
                     brucei|protein GI:38073253], bitscore:855;
                     50pc ID to argonaute-like protein [Trypanosoma brucei
                     brucei strain 927/4 GUTat10.1|protein GI:71748496],
                     bitscore:855;
                     50pc ID to argonaute-like protein, putative [Trypanosoma
                     brucei gambiense DAL972|protein GI:261333222],
                     bitscore:854;
                     48pc ID to argonaute-like protein [Trypanosoma vivax
                     Y486|protein GI:340057669], bitscore:830;
                     32pc ID to argonaute 1 [Crithidia fasciculata|protein
                     GI:189342141], bitscore:358;
                     31pc ID to hypothetical protein, unknown function
                     [Leishmania braziliensis MHOM/BR/75/M2904|protein
                     GI:154333428], bitscore:347;
                     31pc ID to argonaute 1 [Leishmania braziliensis|protein
                     GI:206941165], bitscore:346;
                     31pc ID to argonaute 1 [Leishmania guyanensis|protein
                     GI:226290809], bitscore:345;
                     31pc ID to hypothetical protein, unknown function
                     [Leishmania guyanensis|protein GI:523565755],
                     bitscore:345;
                     30pc ID to aubergine [Angomonas deanei|protein
                     GI:528230204], bitscore:307;
                     32pc ID to argonaute-like protein [Angomonas
                     deanei|protein GI:528229477], bitscore:268;
                     32pc ID to argonaute-like protein [Angomonas
                     deanei|protein GI:528271509], bitscore:263;
                     35pc ID to hypothetical protein LBRM_29_0560 [Leishmania
                     braziliensis MHOM/BR/75/M2904|protein GI:154340902],
                     bitscore:134;
                     39pc ID to hypothetical protein AGDE_04159 [Angomonas
                     deanei|protein GI:528265363], bitscore:106"
                     /codon_start=1
                     /product="putative argonaute-like protein"
                     /protein_id="XP_009312127.1"
                     /db_xref="GeneID:20384129"
                     /db_xref="InterPro:IPR003100"
                     /db_xref="InterPro:IPR003165"
                     /db_xref="InterPro:IPR012337"
                     /db_xref="PFAM:PF02170"
                     /db_xref="PFAM:PF02171"
                     /translation="

MSSRGGRGQDEGGNRGGERGGRGRGEGGYRGGERGGRGRGEGGYRGGERGGRGRGVFGGGGAGRANQLREKDVITAFAQGKKYDCPLEAWSNFLPFYTDREKVIFIYLVSYEMKDGAEVLVDRVKEKACEELLRKMKKETGEKTSFDFAQCVCTGQAVLSPERLPIEEFTYEXKRGRRPKQSHYVVRISELKPAVLQVEEHRTEINTIIGKAVQVCYKEKNGSKYIDLSQGRESSSGRIATFDGVIPKVCTATIKGKEMTVLQIDTCVVVASAQNCLQVIEDIKRREQKNFKRGVMERFVKAKVCTMLNGGKGDLYTVLDVAETKAGDPANLKKNPQQTFVGYFLERYGMNIDPNQTVFRCRTASGKTVCIPPEVLYEMSVSDADKRQLPQLCSLYPNDRAERVKRVLRRLETEENGKAVKFLKAFGLSFGNKFLFVNGRVLKPVEVLIPKGSGYKSVQTLGDSNQQGFVKELRDLKHPGERQTLDFVVYDETRRGEALLNTLSKHLDTMNAGVRLGRAHYINRIGEVKNTPQKNVLGFAFLKVPEKDSYRALKAAWAKRGIVSQVVAKDLSDGRDAPIVMAVSQQVSAKDGKLNWVLDVNKLCPSLSSKTSKLGGILVIGADIGSEQQNIRTETKSKKQDFYTVAFVAFYVRGSEWSPYSNHYQVNGRQQTVFASNRDAEEMSSTEDGGVAVPSEVLSEKIKVFLPEARRHFADKGLNTSAILILRGCASEGELVNAKQHDAAFLGGALKGNLPCAVVAAQRYQHTRFGANTPSEPNVLCNVPRGFVTEEATDKKFGEAFFLNGANCTLGHARSTLYAVLHRTDSFELRELQSLLYGMGFLYPNKTDGLPLPLPLKCAAEYARKFVGLREVQVMPESMRTKLHFL"

     misc_feature    820..1185
                     /locus_tag="DQ04_04891000"
                     /note="PAZ domain, named PAZ after the proteins Piwi
                     Argonaut and Zwille. PAZ is found in two families of
                     proteins that are essential components of RNA-mediated
                     gene-silencing pathways, including RNA interference, the
                     piwi and Dicer families. PAZ functions as a...; Region:
                     PAZ; cl00301"
                     /db_xref="CDD:469713"
     misc_feature    1195..1332
                     /locus_tag="DQ04_04891000"
                     /note="Argonaute linker 2 domain; Region: ArgoL2;
                     pfam16488"
                     /db_xref="CDD:465136"
     misc_feature    1633..2592
                     /locus_tag="DQ04_04891000"
                     /note="This domain is found in the protein Piwi and its
                     relatives; Region: Piwi; smart00950"
                     /db_xref="CDD:214930"
     misc_feature    order(1648..1650,1660..1662,1693..1701,1708..1710,
                     1735..1737,1744..1746,1756..1758,1768..1770)
                     /locus_tag="DQ04_04891000"
                     /note="5' RNA guide strand anchoring site [active]"
                     /db_xref="CDD:239208"
     misc_feature    order(1867..1869,1873..1875,2182..2184,2590..2592)
                     /locus_tag="DQ04_04891000"
                     /note="active site"
                     /db_xref="CDD:239208"
ORIGIN      

atgtcaagcagaggcggtcgtggccaagacgaaggtggaaaccgcggtggtgagagaggcggtcgtggccgaggcgaaggtggataccgcggtggtgagagaggcggtcgtggccgaggcgaaggtggataccgcggtggcgaaagaggtggtcgtggccgcggtgtatttggtggtggcggtgccggccgagccaaccagctacgggaaaaggacgtcataacagcatttgcacaaggcaaaaaatatgattgtcctttggaagcgtggagtaacttccttccattctatacagaccgcgagaaggtaatctttatttaccttgtctcgtatgagatgaaagatggcgctgaagttttggtggatcgcgtaaaggaaaaggcttgtgaagagttgcttcgcaagatgaaaaaagaaaccggtgaaaaaaccagttttgacttcgctcagtgtgtgtgcactgggcaagccgtattatccccggagcggctgcccatagaagagtttacttatgaattnaagaggggacgccgtccaaaacagtcgcactatgtggtgcgaatttctgaactaaagcctgctgtcttgcaagttgaggagcaccgaacggaaataaacaccataatcggcaaagctgtgcaggtttgctacaaagaaaaaaacggaagcaagtacattgatctttctcaaggcagggaaagttcttctggaagaatagcgacgtttgatggagtgatccccaaggtttgtacagctactataaaggggaaagaaatgaccgtgcttcaaattgacacgtgtgtggtagtcgcatccgcgcaaaattgtctgcaggtgattgaagacattaaaaggcgggaacaaaaaaactttaaaaggggtgtcatggagcgttttgttaaagcgaaagtgtgcacgatgctgaacggtggtaaaggggatctttacaccgtgttggatgtcgcggaaaccaaggcgggagatcctgccaatttgaaaaaaaatccacagcaaacatttgttgggtattttttggaacgctatgggatgaacatagatcccaaccagacagtcttccgctgccgtacagcaagtggaaagacagtctgtattccacccgaagttctttacgaaatgtcggttagcgatgccgacaaacgccaactcccccaactttgctcgttgtatcccaatgaccgtgccgaacgagtgaagcgggttctccgtcgtcttgagacggaagaaaatggaaaggccgtgaaattcttgaaggcttttgggctttcttttgggaataagtttttattcgtcaatggccgtgttttaaaaccggtagaggtacttattccaaaaggaagcggctacaaatctgtccaaaccctcggggacagcaatcaacaagggtttgtaaaggaacttcgggatttaaagcatcccggagaaagacaaactttggactttgtggtctacgatgaaacacggagaggcgaggcccttctgaatacactcagtaaacatttggacaccatgaacgccggcgttcgattgggacgggcacattatatcaatcgaatcggggaggtgaaaaacaccccacagaaaaatgttcttgggtttgcttttttaaaagtgcctgagaaggacagctaccgcgccttgaaggcggcgtgggcaaagaggggtattgtaagccaagtggtggcaaaagatttatctgacggtcgggatgccccaattgtgatggctgtttctcaacaagtcagcgcgaaggatggaaagttgaattgggtgctggacgtgaataaactctgtccgtctttgtcgtccaagacttccaagctcggtggaattctggtaattggagctgatattggcagtgagcagcagaatatacgaactgaaaccaagtcaaagaaacaagatttttacacggtcgccttcgtggcgttttatgtgcggggatcagagtggtctccgtacagtaaccactaccaggtgaacgggcggcagcaaacagtttttgcgagcaaccgtgacgcagaggaaatgtcatcgaccgaagatggaggggtggccgtgccatccgaagtcctttcggaaaaaataaaagtctttttgccggaagccaggcgacactttgctgataaaggcttgaacacgtcagcaatattaatcctccgcggctgtgcatcagagggggagctggtaaatgcgaaacagcacgatgctgcctttttgggtggcgctcttaaagggaacttgccttgtgctgtcgtggcagcgcaacgttaccaacacacacgctttggtgcaaatactccaagtgagccaaatgttttgtgcaatgtcccgcgaggtttcgtgaccgaagaggccactgataaaaaatttggcgaggcattcttcttgaatggcgcaaactgcacccttgggcacgcgcgttcaacactgtacgccgtccttcatcggacggattcattcgaattaagggagctacaatcactcttatatggcatgggctttttgtatcccaacaaaacggatgggctgccgctgccgctgcccttgaagtgcgcagcagagtacgctcgaaagtttgttggcctgcgggaggtgcaggttatgcccgagagcatgcgcacaaagttacactttctctag

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