LOCUS       NM_001004877            3687 bp    mRNA    linear   VRT 22-JUN-2025
DEFINITION  Xenopus tropicalis argonaute 2, RISC catalytic component (ago2),
            mRNA.
ACCESSION   NM_001004877
VERSION     NM_001004877.1
KEYWORDS    RefSeq.
SOURCE      Xenopus tropicalis (tropical clawed frog)
  ORGANISM  Xenopus tropicalis
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Amphibia; Batrachia; Anura; Pipoidea; Pipidae; Xenopodinae;
            Xenopus; Silurana.
REFERENCE   1  (bases 1 to 3687)
  AUTHORS   Klein,S.L., Strausberg,R.L., Wagner,L., Pontius,J., Clifton,S.W.
            and Richardson,P.
  TITLE     Genetic and genomic tools for Xenopus research: The NIH Xenopus
            initiative
  JOURNAL   Dev Dyn 225 (4), 384-391 (2002)
   PUBMED   12454917
COMMENT     PROVISIONAL REFSEQ: This record has not yet been subject to final
            NCBI review. The reference sequence was derived from BC075263.1.
            
            ##Evidence-Data-START##
            Transcript exon combination :: BC075263.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMD00028290, SAMD00028291
                                           [ECO:0000348]
            ##Evidence-Data-END##
FEATURES             Location/Qualifiers
     source          1..3687
                     /organism="Xenopus tropicalis"
                     /mol_type="mRNA"
                     /db_xref="taxon:8364"
                     /chromosome="6"
                     /map="6"
     gene            1..3687
                     /gene="ago2"
                     /gene_synonym="Argonaute2; eif2c2"
                     /note="argonaute 2, RISC catalytic component"
                     /db_xref="GeneID:448205"
                     /db_xref="Xenbase:XB-GENE-1001916"
     exon            1..260
                     /gene="ago2"
                     /gene_synonym="Argonaute2; eif2c2"
                     /inference="alignment:Splign:2.1.0"
     misc_feature    122..124
                     /gene="ago2"
                     /gene_synonym="Argonaute2; eif2c2"
                     /note="upstream in-frame stop codon"
     CDS             239..2854
                     /gene="ago2"
                     /gene_synonym="Argonaute2; eif2c2"
                     /EC_number="3.1.26.n2"
                     /note="argonaute RISC catalytic component 2"
                     /codon_start=1
                     /product="protein argonaute-2"
                     /protein_id="NP_001004877.1"
                     /db_xref="GeneID:448205"
                     /db_xref="Xenbase:XB-GENE-1001916"
                     /translation="

MYAGAGPVLVPPTPTPPLPMPAYTFKPPPRPDFGTSGRTIKLQANFFEMDIPKIEIYHYEIDIKPEKCPRRVNREIVEHMVQHFKAQIFGDRKPVFDGRKNLYTAMPLPIARDKQVELEVTLPGEGKDRIFKVAIKWMACVSLQALHDALSGRLPSVPFETIQALDVVMRHLPSMRYTPVGRSFFTASEGCANPLGGGREVWFGFHQSVRPSLWKMMLNIDVSATAFYKAQPVIEFMCEVLDFKSIEEQQKPLTDSQRVKFTKEIKGLKVEITHCGQMKRKYRVCNVTRRPASHQTFPLQQESGQTVECTVAQYFKDRHKLVLRYPHLPCLQVGQEQKHTYLPLEVCNIVAGQRCIKKLTDNQTSTMIRATARSAPDRQEEISKLMRSASFNTDPFVREFGIMVKDDMTDVTGRVLQPPSILYGGRVWEEPNAPLNKAIATPVQGVWDMRNKQFHTGIEIKVWAIACFAPQRQCTEVHLKTFTEQLRKISRDAGMPIQGQPCFCKYAQGADSVEPMFRHLKNTYTGLQLVVVILPGKTPVYAEVKRVGDTVLGMATQCVQMKNVQRTTPQTLSNLCLKINVKLGGVNNILLPQGRPPVFQQPVIFLGADVTHPPAGDGKKPSIAAVVGSMDAHPNRYCATVRVQQHRQEIIQDLSAMVRELLIQFYKSTRFKPTRIIFYRDGVSEGQFQQVLHHELLAIREACIKLEKDYQPGITFIVVQKRHHTRLFCTDRNERVGKSGNIPAGTTVDTKITHPSEFDFYLCSHAGIQGTSRPSHYHVLWDDNRFSSDELQILTYQLCHTYVRCTRSVSIPAPAYYAHLVAFRARYHLVDKEHDSAEGSHTSGQSNGRDQQALAKAVQVHQDTLRTMYFA"

     misc_feature    485..745
                     /gene="ago2"
                     /gene_synonym="Argonaute2; eif2c2"
                     /note="N-terminal domain of argonaute; Region: ArgoN;
                     pfam16486"
                     /db_xref="CDD:465134"
     misc_feature    773..925
                     /gene="ago2"
                     /gene_synonym="Argonaute2; eif2c2"
                     /note="Argonaute linker 1 domain; Region: ArgoL1;
                     pfam08699"
                     /db_xref="CDD:462567"
     misc_feature    926..1288
                     /gene="ago2"
                     /gene_synonym="Argonaute2; eif2c2"
                     /note="PAZ domain, argonaute_like subfamily. Argonaute is
                     part of the RNA-induced silencing complex (RISC), and is
                     an endonuclease that plays a key role in the RNA
                     interference pathway. The PAZ domain has been named after
                     the proteins Piwi,Argonaut, and Zwille; Region:
                     PAZ_argonaute_like; cd02846"
                     /db_xref="CDD:239212"
     misc_feature    order(1082..1084,1127..1129,1169..1171,1181..1183,
                     1235..1237,1256..1258,1262..1264)
                     /gene="ago2"
                     /gene_synonym="Argonaute2; eif2c2"
                     /note="nucleic acid-binding interface [nucleotide
                     binding]; other site"
                     /db_xref="CDD:239212"
     misc_feature    1178..1195
                     /gene="ago2"
                     /gene_synonym="Argonaute2; eif2c2"
                     /note="propagated from UniProtKB/Swiss-Prot (Q6DJB9.1);
                     Region: Interaction with guide RNA. /evidence=ECO:0000250"
     misc_feature    1421..2725
                     /gene="ago2"
                     /gene_synonym="Argonaute2; eif2c2"
                     /note="PIWI domain, Argonaute-like subfamily. Argonaute is
                     the central component of the RNA-induced silencing complex
                     (RISC) and related complexes. The PIWI domain is the
                     C-terminal portion of Argonaute and consists of two
                     subdomains, one of which provides the...; Region:
                     Piwi_ago-like; cd04657"
                     /db_xref="CDD:240015"
     misc_feature    1844..1972
                     /gene="ago2"
                     /gene_synonym="Argonaute2; eif2c2"
                     /note="propagated from UniProtKB/Swiss-Prot (Q6DJB9.1);
                     Region: Interaction with guide RNA. /evidence=ECO:0000250"
     misc_feature    order(1859..1861,1871..1873,1907..1918,1925..1927,
                     1949..1951,1958..1960,1970..1972,1982..1984)
                     /gene="ago2"
                     /gene_synonym="Argonaute2; eif2c2"
                     /note="5' RNA guide strand anchoring site [active]"
                     /db_xref="CDD:240015"
     misc_feature    2033..2044
                     /gene="ago2"
                     /gene_synonym="Argonaute2; eif2c2"
                     /note="propagated from UniProtKB/Swiss-Prot (Q6DJB9.1);
                     Region: Interaction with GW182 family members.
                     /evidence=ECO:0000255"
     misc_feature    order(2063..2065,2069..2071,2279..2281,2693..2695)
                     /gene="ago2"
                     /gene_synonym="Argonaute2; eif2c2"
                     /note="active site"
                     /db_xref="CDD:240015"
     misc_feature    2222..2254
                     /gene="ago2"
                     /gene_synonym="Argonaute2; eif2c2"
                     /note="propagated from UniProtKB/Swiss-Prot (Q6DJB9.1);
                     Region: Interaction with GW182 family members.
                     /evidence=ECO:0000255"
     misc_feature    2399..2404
                     /gene="ago2"
                     /gene_synonym="Argonaute2; eif2c2"
                     /note="propagated from UniProtKB/Swiss-Prot (Q6DJB9.1);
                     Region: Interaction with guide RNA. /evidence=ECO:0000250"
     misc_feature    2531..2557
                     /gene="ago2"
                     /gene_synonym="Argonaute2; eif2c2"
                     /note="propagated from UniProtKB/Swiss-Prot (Q6DJB9.1);
                     Region: Interaction with guide RNA. /evidence=ECO:0000250"
     misc_feature    2642..2710
                     /gene="ago2"
                     /gene_synonym="Argonaute2; eif2c2"
                     /note="propagated from UniProtKB/Swiss-Prot (Q6DJB9.1);
                     Region: Interaction with guide RNA. /evidence=ECO:0000250"
     misc_feature    2738..2806
                     /gene="ago2"
                     /gene_synonym="Argonaute2; eif2c2"
                     /note="propagated from UniProtKB/Swiss-Prot (Q6DJB9.1);
                     Region: Disordered. /evidence=ECO:0000256|SAM:MobiDB-lite"
     exon            261..459
                     /gene="ago2"
                     /gene_synonym="Argonaute2; eif2c2"
                     /inference="alignment:Splign:2.1.0"
     exon            460..580
                     /gene="ago2"
                     /gene_synonym="Argonaute2; eif2c2"
                     /inference="alignment:Splign:2.1.0"
     exon            581..765
                     /gene="ago2"
                     /gene_synonym="Argonaute2; eif2c2"
                     /inference="alignment:Splign:2.1.0"
     exon            766..902
                     /gene="ago2"
                     /gene_synonym="Argonaute2; eif2c2"
                     /inference="alignment:Splign:2.1.0"
     exon            903..1037
                     /gene="ago2"
                     /gene_synonym="Argonaute2; eif2c2"
                     /inference="alignment:Splign:2.1.0"
     exon            1038..1125
                     /gene="ago2"
                     /gene_synonym="Argonaute2; eif2c2"
                     /inference="alignment:Splign:2.1.0"
     exon            1126..1273
                     /gene="ago2"
                     /gene_synonym="Argonaute2; eif2c2"
                     /inference="alignment:Splign:2.1.0"
     exon            1274..1393
                     /gene="ago2"
                     /gene_synonym="Argonaute2; eif2c2"
                     /inference="alignment:Splign:2.1.0"
     exon            1394..1543
                     /gene="ago2"
                     /gene_synonym="Argonaute2; eif2c2"
                     /inference="alignment:Splign:2.1.0"
     exon            1544..1677
                     /gene="ago2"
                     /gene_synonym="Argonaute2; eif2c2"
                     /inference="alignment:Splign:2.1.0"
     exon            1678..1862
                     /gene="ago2"
                     /gene_synonym="Argonaute2; eif2c2"
                     /inference="alignment:Splign:2.1.0"
     exon            1863..2022
                     /gene="ago2"
                     /gene_synonym="Argonaute2; eif2c2"
                     /inference="alignment:Splign:2.1.0"
     exon            2023..2113
                     /gene="ago2"
                     /gene_synonym="Argonaute2; eif2c2"
                     /inference="alignment:Splign:2.1.0"
     exon            2114..2308
                     /gene="ago2"
                     /gene_synonym="Argonaute2; eif2c2"
                     /inference="alignment:Splign:2.1.0"
     exon            2309..2443
                     /gene="ago2"
                     /gene_synonym="Argonaute2; eif2c2"
                     /inference="alignment:Splign:2.1.0"
     exon            2444..2545
                     /gene="ago2"
                     /gene_synonym="Argonaute2; eif2c2"
                     /inference="alignment:Splign:2.1.0"
     exon            2546..2745
                     /gene="ago2"
                     /gene_synonym="Argonaute2; eif2c2"
                     /inference="alignment:Splign:2.1.0"
     exon            2746..3670
                     /gene="ago2"
                     /gene_synonym="Argonaute2; eif2c2"
                     /inference="alignment:Splign:2.1.0"
ORIGIN      

ctcagttcatgtcgcgcaaaagaaagagaaaaaaaaggaaagaagcgagagaagcgagaggcgggaactcggcgtattccctcccacacaccgtgccttacaccatggagccctaacggactgactggccgctctcggaggggttctgtgcacagcgaagggggcgggactggctgcgttaccccctgtgtctggaactttattttccccctcgcacttcgctcttggggccgcagacatgtacgccggggccgggccggtgctcgttccaccaaccccaacaccacctctcccaatgccggcgtataccttcaagcctccgccacggccagattttggtacctcagggaggaccatcaagctccaggccaacttttttgaaatggacattcccaaaattgaaatatatcactatgagattgatataaaaccagagaaatgccccagacgagtaaacagggaaatagttgaacacatggtccagcactttaaagctcaaatcttcggggatcgaaaaccagtgtttgatggaagaaaaaacctttacactgcaatgcctcttccgattgcaagagataaacaggtggagttagaagtcacactacctggggaagggaaagatcgcatcttcaaagttgcaatcaagtggatggcgtgtgtaagcttgcaggccctacatgatgcactgtctggtcgtcttcccagtgtcccttttgagacaattcaggctttagatgttgtgatgaggcatttaccttctatgaggtatacaccagttggtcgttctttttttactgcatctgaaggctgtgcaaatcctctcgggggtggcagagaagtttggtttggatttcatcagtccgtacggccgtcgctctggaaaatgatgctcaatatcgatgtgtctgctacagcattttacaaagcacaaccagtaattgagtttatgtgtgaagttctggattttaaaagcatcgaggagcaacagaaacctctgactgattcccaaagggttaaatttaccaaagaaattaaaggtttaaaagtggaaataactcactgtggacaaatgaaacggaaatacagagtttgcaacgtaacaaggcgaccagcaagtcatcaaacattccctctccagcaggagagtggacagacggtagaatgcaccgtcgcacagtactttaaggacagacacaaactagttctgcgttaccctcatttaccatgtttacaggtcgggcaggagcagaaacacacatacctcccacttgaggtatgcaatatagtggctggtcaaaggtgcataaagaaacttacggacaatcagacctccactatgatacgggcaactgctcgatctgctccagatcgccaagaagagatcagtaaattaatgcgaagtgcaagttttaatactgatccctttgtgcgagagtttggtataatggtgaaagatgacatgaccgatgtcacaggcagagtccttcaacctccttcaatcctttatggaggcagggtatgggaagagccaaatgctcccttgaacaaagcaattgccacccctgtccagggtgtctgggacatgagaaacaaacagttccacaccggcattgaaataaaggtgtgggccatcgcctgctttgccccacaacggcagtgtactgaagttcacctcaagacatttacagaacagttgaggaagatctcacgtgatgccgggatgcctatccagggacagccgtgtttctgcaaatatgcccagggagcagatagcgttgagccaatgttcaggcatctgaagaatacttatacagggctacaactagtcgttgtaatacttcctgggaaaacccctgtctatgcggaagtgaaacgtgtgggggacactgttctgggaatggctactcagtgtgttcagatgaaaaatgtgcagaggaccacaccacaaaccttgtctaacctgtgcttaaaaatcaatgtaaaattaggaggcgtcaataacatcttgttgccccagggcagacctcctgtgtttcaacagcctgtcatatttcttggagctgatgttacccacccaccagcaggggacggcaaaaagccttcaattgctgcggttgtaggtagtatggatgcacaccccaaccgatactgtgccactgtgagggtacagcagcacaggcaagaaatcatccaggatttgtctgccatggttcgagagctgcttatccagttctacaagtcaacacgcttcaagcccacaagaatcatcttttacagagacggtgtctcggaaggacagtttcaacaggtgctccatcatgagcttctggccattagggaagcctgtattaaactggagaaggattaccagccgggaattacatttattgtagtgcaaaaaaggcatcacacacggctattctgtacagaccgaaatgagcgagtagggaagagtggaaatataccagccggtaccaccgtagacacaaagatcacccacccatcagagtttgatttttacctgtgcagtcacgctggtattcagggaacaagcaggccgtcgcactatcatgtcctgtgggatgacaatcgcttttcatcggatgagctgcagatcctcacttaccagctctgccacacttatgtccgttgcactcgctctgtatctatccctgctccagcatactatgctcacctggtggcattcagagcaaggtatcatttggtggataaagagcatgacagtgctgaagggagccatacatccggtcagagtaacgggagagatcagcaagcacttgctaaggcagtgcaggtacatcaggacacactgcgtacgatgtactttgcgtaacaggcgctgttatccagtgacttaatagcactcctggaaacggattcacacagaccagctgcacttagttgtttgtaaaatagtccatactacggcgactcccagcaaaaagtgcaatctaccggtaaccctgttgtttcccccactgtccaaccagccttccgtccgaaccgcttctcacattatgtttccattcacgatgcagacctgttgcgattaatgtagcttggagtttgcttggtatgggaatgtttacaaaggtgcagagagagaaacacgtacccatgtagaaaaactgaacacgtaacctcagggctttagacttttgctatggaataatgtttcggatcctgtattatccctgttcagttgctgaatttaatgtgatggttgctgtcagttgggttggttaaaaaaaaaggagctaaaaaaagagcttaaaaagaaaaaaaaggcattagcagaacatttgaagccttaaaatattgatattttttaattagcaaacgattttaccatatgtagctttttatgtgtcaaatgttctggtacataactctatgtacttcagagagtcagtcatattaatgaatttaatcatgactggagaggattagtgccctagtgtctccatctcatgcttttacatatattattacccggtgccattcagaacacactctccatagaaagtctcgtttatcgtatttgtgtctaaactctaacctttccaaagttgcttaccaatacgggtctgtggcaatcagtacaatcatgttaagctaatgtaaaaagaaaagaaaaacacaaaaaaaaaaaaaaaaaaaaaaaaa

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