LOCUS       NM_001160028            3062 bp    mRNA    linear   VRT 05-JUN-2025
DEFINITION  Danio rerio argonaute RISC catalytic component 3b (ago3b), mRNA.
ACCESSION   NM_001160028 XM_691471
VERSION     NM_001160028.1
KEYWORDS    RefSeq.
SOURCE      Danio rerio (zebrafish)
  ORGANISM  Danio rerio
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Actinopterygii; Neopterygii; Teleostei; Ostariophysi;
            Cypriniformes; Danionidae; Danioninae; Danio.
REFERENCE   1  (bases 1 to 3062)
  AUTHORS   Raman,R., Ryon,M. and Jagadeeswaran,P.
  TITLE     RNaseH-mediated simultaneous piggyback knockdown of multiple genes
            in adult zebrafish
  JOURNAL   Sci Rep 10 (1), 20187 (2020)
   PUBMED   33214638
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3062)
  AUTHORS   Liu,Y., Zhu,Z., Ho,I.H.T., Shi,Y., Li,J., Wang,X., Chan,M.T.V. and
            Cheng,C.H.K.
  TITLE     Genetic Deletion of miR-430 Disrupts Maternal-Zygotic Transition
            and Embryonic Body Plan
  JOURNAL   Front Genet 11, 853 (2020)
   PUBMED   32849832
  REMARK    Publication Status: Online-Only
REFERENCE   3  (bases 1 to 3062)
  AUTHORS   Qiu,W., Liu,S., Yang,F., Dong,P., Yang,M., Wong,M. and Zheng,C.
  TITLE     Metabolism disruption analysis of zebrafish larvae in response to
            BPA and BPA analogs based on RNA-Seq technique
  JOURNAL   Ecotoxicol Environ Saf 174, 181-188 (2019)
   PUBMED   30826544
REFERENCE   4  (bases 1 to 3062)
  AUTHORS   Bayes,A., Collins,M.O., Reig-Viader,R., Gou,G., Goulding,D.,
            Izquierdo,A., Choudhary,J.S., Emes,R.D. and Grant,S.G.
  TITLE     Evolution of complexity in the zebrafish synapse proteome
  JOURNAL   Nat Commun 8, 14613 (2017)
   PUBMED   28252024
  REMARK    Publication Status: Online-Only
REFERENCE   5  (bases 1 to 3062)
  AUTHORS   Katz,S., Cussigh,D., Urban,N., Blomfield,I., Guillemot,F.,
            Bally-Cuif,L. and Coolen,M.
  TITLE     A Nuclear Role for miR-9 and Argonaute Proteins in Balancing
            Quiescent and Activated Neural Stem Cell States
  JOURNAL   Cell Rep 17 (5), 1383-1398 (2016)
   PUBMED   27783951
REFERENCE   6  (bases 1 to 3062)
  AUTHORS   Elkon,R., Milon,B., Morrison,L., Shah,M., Vijayakumar,S.,
            Racherla,M., Leitch,C.C., Silipino,L., Hadi,S., Weiss-Gayet,M.,
            Barras,E., Schmid,C.D., Ait-Lounis,A., Barnes,A., Song,Y.,
            Eisenman,D.J., Eliyahu,E., Frolenkov,G.I., Strome,S.E., Durand,B.,
            Zaghloul,N.A., Jones,S.M., Reith,W. and Hertzano,R.
  TITLE     RFX transcription factors are essential for hearing in mice
  JOURNAL   Nat Commun 6, 8549 (2015)
   PUBMED   26469318
  REMARK    Publication Status: Online-Only
REFERENCE   7  (bases 1 to 3062)
  AUTHORS   McFarlane,L., Svingen,T., Braasch,I., Koopman,P., Schartl,M. and
            Wilhelm,D.
  TITLE     Expansion of the Ago gene family in the teleost clade
  JOURNAL   Dev Genes Evol 221 (2), 95-104 (2011)
   PUBMED   21556854
REFERENCE   8  (bases 1 to 3062)
  AUTHORS   Cifuentes,D., Xue,H., Taylor,D.W., Patnode,H., Mishima,Y.,
            Cheloufi,S., Ma,E., Mane,S., Hannon,G.J., Lawson,N.D., Wolfe,S.A.
            and Giraldez,A.J.
  TITLE     A novel miRNA processing pathway independent of Dicer requires
            Argonaute2 catalytic activity
  JOURNAL   Science 328 (5986), 1694-1698 (2010)
   PUBMED   20448148
COMMENT     PROVISIONAL REFSEQ: This record has not yet been subject to final
            NCBI review. The reference sequence was derived from
            JBMGRA010000019.1.
            
            On May 10, 2009 this sequence version replaced XM_691471.3.
            
            ##Evidence-Data-START##
            Transcript exon combination :: GDQH01005553.1, SRR26395039.549515.1
                                           [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA898400, SAMEA898403
                                           [ECO:0000348]
            ##Evidence-Data-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-498               JBMGRA010000019.1  11758858-11759355
            499-670             JBMGRA010000019.1  11764528-11764699
            671-791             JBMGRA010000019.1  11764865-11764985
            792-1000            JBMGRA010000019.1  11766096-11766304
            1001-1137           JBMGRA010000019.1  11766525-11766661
            1138-1272           JBMGRA010000019.1  11768885-11769019
            1273-1360           JBMGRA010000019.1  11769832-11769919
            1361-1508           JBMGRA010000019.1  11770413-11770560
            1509-1628           JBMGRA010000019.1  11771537-11771656
            1629-1751           JBMGRA010000019.1  11771764-11771886
            1752-1885           JBMGRA010000019.1  11774425-11774558
            1886-2070           JBMGRA010000019.1  11774646-11774830
            2071-2230           JBMGRA010000019.1  11775388-11775547
            2231-2321           JBMGRA010000019.1  11775642-11775732
            2322-2516           JBMGRA010000019.1  11778576-11778770
            2517-2651           JBMGRA010000019.1  11779898-11780032
            2652-2753           JBMGRA010000019.1  11780121-11780222
            2754-2953           JBMGRA010000019.1  11780893-11781092
            2954-3062           JBMGRA010000019.1  11782643-11782751
FEATURES             Location/Qualifiers
     source          1..3062
                     /organism="Danio rerio"
                     /mol_type="mRNA"
                     /strain="Tuebingen"
                     /db_xref="taxon:7955"
                     /chromosome="19"
                     /map="19"
     gene            1..3062
                     /gene="ago3b"
                     /gene_synonym="ago3; Argonaute3; eif2c3; eif2c3b;
                     sb:eu332; si:dkey-3n22.3"
                     /note="argonaute RISC catalytic component 3b"
                     /db_xref="GeneID:568159"
                     /db_xref="ZFIN:ZDB-GENE-060503-452"
     exon            1..498
                     /gene="ago3b"
                     /gene_synonym="ago3; Argonaute3; eif2c3; eif2c3b;
                     sb:eu332; si:dkey-3n22.3"
                     /inference="alignment:Splign:2.1.0"
     misc_feature    477..479
                     /gene="ago3b"
                     /gene_synonym="ago3; Argonaute3; eif2c3; eif2c3b;
                     sb:eu332; si:dkey-3n22.3"
                     /note="upstream in-frame stop codon"
     CDS             480..3062
                     /gene="ago3b"
                     /gene_synonym="ago3; Argonaute3; eif2c3; eif2c3b;
                     sb:eu332; si:dkey-3n22.3"
                     /EC_number="3.1.26.n2"
                     /note="argonaute 3b; eukaryotic translation initiation
                     factor 2C, 3b; argonaute RISC catalytic component 3"
                     /codon_start=1
                     /product="protein argonaute-3"
                     /protein_id="NP_001153500.1"
                     /db_xref="GeneID:568159"
                     /db_xref="ZFIN:ZDB-GENE-060503-452"
                     /translation="

MEIGTTGAVGAAPQFSVPRRPGYGTMGKPIKLLANCFQVDIPKMDVYLYDVDIKPEKCPRRVNREVVDSMVQHFKVTIFGDRRPVYDGKKSLYTAQPLPVASAGVDLDVTLPGEGGKDRIFKVTIKFVSLVSWHMLHEVLTGRSTPDPLELDKPISTNPVHAVDVVLRHLPSMRYTPVGRSFFSSPEGYDHPLGGGREVWFGFHQSVRPAMWKMMLNIDVSATAFYKAQPVIQFMCEVLDIHNIDEQPRPLTDSHRVKFTKEIKGLKVEVTHCGTMRRKYRVCNVTRRPASHQTFPLQLENGQTVERTVAQYFREKYNLQLKYPHLPCLQVGQEQKHTYLPLEVCNIVAGQRCIKKLTDNQTSTMIKATARSAPDRQEEISRLVRSANYNSDPFVQEFQFRVRDEMAEVTGRVLPAPMLQYGGRNRTVATPSHGVWDMRGKQFHTGVEIKMWAIACFATQRQCREEVLKGFTDQLRKISKDAGMPIQGQPCFCKYAQGADNVEPMFRHLKNTYAGLQLIIVILPGKTPVYAEVKRVGDTLLGMATQCVQVKNVVKTSPQTLSNLCLKINVKLGGINNILVPHQRPSVFQQPVIFLGADVTHPPAGDGKKPSIAAVVGSMDAHPSRYCATVRVQRPRQEVIQDLASMVRELLIQFYKSTHYKPTRIIFYRDGVSEGQFRQVLYYELLAIREACISLEKEYQPGITYIVVQKRHHTRLFCADRAERVGRSGNIPAGTTVDTDITHPYEFDFYLCSHAGIQGTSRPSHYYVLWDDNCFTADEFQLLTYQLCHTYVRCTRSVSIPAPAYYAHLVAFRARYHLVDKEHDSAEGSHVSGQSNGRDPQALAKAVQIHHDTLRTMYFA"

     misc_feature    696..980
                     /gene="ago3b"
                     /gene_synonym="ago3; Argonaute3; eif2c3; eif2c3b;
                     sb:eu332; si:dkey-3n22.3"
                     /note="N-terminal domain of argonaute; Region: ArgoN;
                     pfam16486"
                     /db_xref="CDD:465134"
     misc_feature    1008..1160
                     /gene="ago3b"
                     /gene_synonym="ago3; Argonaute3; eif2c3; eif2c3b;
                     sb:eu332; si:dkey-3n22.3"
                     /note="Argonaute linker 1 domain; Region: ArgoL1;
                     pfam08699"
                     /db_xref="CDD:462567"
     misc_feature    1161..1523
                     /gene="ago3b"
                     /gene_synonym="ago3; Argonaute3; eif2c3; eif2c3b;
                     sb:eu332; si:dkey-3n22.3"
                     /note="PAZ domain, argonaute_like subfamily. Argonaute is
                     part of the RNA-induced silencing complex (RISC), and is
                     an endonuclease that plays a key role in the RNA
                     interference pathway. The PAZ domain has been named after
                     the proteins Piwi,Argonaut, and Zwille; Region:
                     PAZ_argonaute_like; cd02846"
                     /db_xref="CDD:239212"
     misc_feature    order(1317..1319,1362..1364,1404..1406,1416..1418,
                     1470..1472,1491..1493,1497..1499)
                     /gene="ago3b"
                     /gene_synonym="ago3; Argonaute3; eif2c3; eif2c3b;
                     sb:eu332; si:dkey-3n22.3"
                     /note="nucleic acid-binding interface [nucleotide
                     binding]; other site"
                     /db_xref="CDD:239212"
     misc_feature    1656..2933
                     /gene="ago3b"
                     /gene_synonym="ago3; Argonaute3; eif2c3; eif2c3b;
                     sb:eu332; si:dkey-3n22.3"
                     /note="PIWI domain, Argonaute-like subfamily. Argonaute is
                     the central component of the RNA-induced silencing complex
                     (RISC) and related complexes. The PIWI domain is the
                     C-terminal portion of Argonaute and consists of two
                     subdomains, one of which provides the...; Region:
                     Piwi_ago-like; cd04657"
                     /db_xref="CDD:240015"
     misc_feature    order(2067..2069,2079..2081,2115..2126,2133..2135,
                     2157..2159,2166..2168,2178..2180,2190..2192)
                     /gene="ago3b"
                     /gene_synonym="ago3; Argonaute3; eif2c3; eif2c3b;
                     sb:eu332; si:dkey-3n22.3"
                     /note="5' RNA guide strand anchoring site [active]"
                     /db_xref="CDD:240015"
     misc_feature    2067..2180
                     /gene="ago3b"
                     /gene_synonym="ago3; Argonaute3; eif2c3; eif2c3b;
                     sb:eu332; si:dkey-3n22.3"
                     /note="propagated from UniProtKB/Swiss-Prot (A3KPK0.1);
                     Region: Interaction with guide RNA.
                     /evidence=ECO:0000250|UniProtKB:Q9H9G7"
     misc_feature    order(2271..2273,2277..2279,2487..2489,2901..2903)
                     /gene="ago3b"
                     /gene_synonym="ago3; Argonaute3; eif2c3; eif2c3b;
                     sb:eu332; si:dkey-3n22.3"
                     /note="active site"
                     /db_xref="CDD:240015"
     misc_feature    2751..2894
                     /gene="ago3b"
                     /gene_synonym="ago3; Argonaute3; eif2c3; eif2c3b;
                     sb:eu332; si:dkey-3n22.3"
                     /note="propagated from UniProtKB/Swiss-Prot (A3KPK0.1);
                     Region: Interaction with guide RNA.
                     /evidence=ECO:0000250|UniProtKB:Q9H9G7"
     exon            499..670
                     /gene="ago3b"
                     /gene_synonym="ago3; Argonaute3; eif2c3; eif2c3b;
                     sb:eu332; si:dkey-3n22.3"
                     /inference="alignment:Splign:2.1.0"
     exon            671..791
                     /gene="ago3b"
                     /gene_synonym="ago3; Argonaute3; eif2c3; eif2c3b;
                     sb:eu332; si:dkey-3n22.3"
                     /inference="alignment:Splign:2.1.0"
     exon            792..1000
                     /gene="ago3b"
                     /gene_synonym="ago3; Argonaute3; eif2c3; eif2c3b;
                     sb:eu332; si:dkey-3n22.3"
                     /inference="alignment:Splign:2.1.0"
     exon            1001..1137
                     /gene="ago3b"
                     /gene_synonym="ago3; Argonaute3; eif2c3; eif2c3b;
                     sb:eu332; si:dkey-3n22.3"
                     /inference="alignment:Splign:2.1.0"
     exon            1138..1272
                     /gene="ago3b"
                     /gene_synonym="ago3; Argonaute3; eif2c3; eif2c3b;
                     sb:eu332; si:dkey-3n22.3"
                     /inference="alignment:Splign:2.1.0"
     exon            1273..1360
                     /gene="ago3b"
                     /gene_synonym="ago3; Argonaute3; eif2c3; eif2c3b;
                     sb:eu332; si:dkey-3n22.3"
                     /inference="alignment:Splign:2.1.0"
     exon            1361..1508
                     /gene="ago3b"
                     /gene_synonym="ago3; Argonaute3; eif2c3; eif2c3b;
                     sb:eu332; si:dkey-3n22.3"
                     /inference="alignment:Splign:2.1.0"
     exon            1509..1628
                     /gene="ago3b"
                     /gene_synonym="ago3; Argonaute3; eif2c3; eif2c3b;
                     sb:eu332; si:dkey-3n22.3"
                     /inference="alignment:Splign:2.1.0"
     exon            1629..1751
                     /gene="ago3b"
                     /gene_synonym="ago3; Argonaute3; eif2c3; eif2c3b;
                     sb:eu332; si:dkey-3n22.3"
                     /inference="alignment:Splign:2.1.0"
     exon            1752..1885
                     /gene="ago3b"
                     /gene_synonym="ago3; Argonaute3; eif2c3; eif2c3b;
                     sb:eu332; si:dkey-3n22.3"
                     /inference="alignment:Splign:2.1.0"
     exon            1886..2070
                     /gene="ago3b"
                     /gene_synonym="ago3; Argonaute3; eif2c3; eif2c3b;
                     sb:eu332; si:dkey-3n22.3"
                     /inference="alignment:Splign:2.1.0"
     exon            2071..2230
                     /gene="ago3b"
                     /gene_synonym="ago3; Argonaute3; eif2c3; eif2c3b;
                     sb:eu332; si:dkey-3n22.3"
                     /inference="alignment:Splign:2.1.0"
     exon            2231..2321
                     /gene="ago3b"
                     /gene_synonym="ago3; Argonaute3; eif2c3; eif2c3b;
                     sb:eu332; si:dkey-3n22.3"
                     /inference="alignment:Splign:2.1.0"
     exon            2322..2516
                     /gene="ago3b"
                     /gene_synonym="ago3; Argonaute3; eif2c3; eif2c3b;
                     sb:eu332; si:dkey-3n22.3"
                     /inference="alignment:Splign:2.1.0"
     exon            2517..2651
                     /gene="ago3b"
                     /gene_synonym="ago3; Argonaute3; eif2c3; eif2c3b;
                     sb:eu332; si:dkey-3n22.3"
                     /inference="alignment:Splign:2.1.0"
     exon            2652..2753
                     /gene="ago3b"
                     /gene_synonym="ago3; Argonaute3; eif2c3; eif2c3b;
                     sb:eu332; si:dkey-3n22.3"
                     /inference="alignment:Splign:2.1.0"
     exon            2754..2953
                     /gene="ago3b"
                     /gene_synonym="ago3; Argonaute3; eif2c3; eif2c3b;
                     sb:eu332; si:dkey-3n22.3"
                     /inference="alignment:Splign:2.1.0"
     exon            2954..3062
                     /gene="ago3b"
                     /gene_synonym="ago3; Argonaute3; eif2c3; eif2c3b;
                     sb:eu332; si:dkey-3n22.3"
                     /inference="alignment:Splign:2.1.0"
ORIGIN      

ttgatcagacgtcaacggccgcaagcgaactcgacaggaataaaagacacacgttttgactcggcgagggatgtgaagatataaacttcaaatctgagcgcatgtctttaaaatataagagcttgtcactttaaaagacacaccgcgaacgcttcgaggctcctgacaaactctgtttcgcgatggctgttagaaatatttccttcggagtcttttggcttgtcagctggtagcaattacggctctacggcatacggcgggagccacggctgaaggtcggtgctttttgcttgactgattcgctacgggctgtgtgaagctcagaacctccgagcggcccgtttcaatacccgcggtagaagccggtgtgtgtgtcggtcgttcggtttacagtggacattcagcggctttacacgaacaccgctcatctggtatttgatcccccgaagccggctagaatccggacagagacctcatgaatggaaatcggaactacaggagccgttggggctgcgccccagttctccgtacctcggaggcctggctatggcaccatggggaagcccatcaagctgctggccaactgctttcaggtggacatccccaagatggatgtctacctgtacgatgtggacatcaagccagaaaagtgcccacgacgtgttaacagggaagtggtggattctatggtgcagcactttaaagtgacaatctttggggacagaagaccagtttatgatggcaagaagagcctttacactgctcagcctttaccagtggcctcagcgggggtggacctggacgtgaccttgccaggtgaaggtgggaaggacaggattttcaaggtaaccattaaatttgtgtcgctggtcagttggcacatgctgcatgaggtcctcactggacggagcactcctgatcctctggagcttgacaagccaatcagcaccaatcccgtacacgccgtagatgttgtcctgcgccatctgccatcaatgaggtacacccctgtgggaaggtcgtttttctcctcccctgagggctatgatcacccactgggcggggggagggaggtgtggtttggcttccaccaatcagtgcgaccggctatgtggaagatgatgctcaacattgatgtgtctgccactgctttctacaaagctcagcctgtgattcagttcatgtgtgaggttcttgatatccacaacatcgatgagcagccccgtcctctcaccgattcccacagagtcaaattcaccaaagagatcaaaggtttaaaggtggaggtcacccactgtggcaccatgaggaggaagtacagggtgtgtaatgttactcgtcgaccagctagccatcagacgtttcctctacagctagaaaatggacagactgtggagagaacagtggcacaatatttcagggaaaagtacaacttacagctcaagtacccccatttgccctgcctgcaagtgggacaggagcagaaacacacatacctacctttagaggtgtgcaacatagtggcaggtcaacgctgcataaaaaaactgaccgacaaccaaacctccaccatgatcaaagccacggcccgctccgcaccggacagacaagaggagatcagcagactggtgcgcagtgccaactataattcagacccatttgtgcaagagttccagttccgtgtgcgggacgagatggcggaggtgacgggtcgagttctgcccgcccccatgctgcagtacggcggcaggaatcgtactgtagccacccctagtcatggagtgtgggacatgagaggcaagcagtttcacactggagtggagatcaagatgtgggccatcgcttgctttgccactcagagacaatgtagagaggaagttctcaagggctttacagaccagctgcggaagatctccaaggatgcagggatgcctattcaggggcagccgtgcttctgcaagtatgctcagggagcagataatgtggagcccatgttccggcacctgaagaacacttatgctggtcttcaactcatcatcgtcattctgcctggaaaaactccagtctacgccgaagtgaaacgtgttggagacactcttctgggaatggccactcagtgcgttcaggtgaagaatgtggtaaagacgtctcctcagaccctttccaacctctgtttgaagatcaacgtcaaactgggcggcattaacaacatcctagtgccccatcagcggccttctgttttccagcagccagtcatatttctgggagctgatgtcactcatccgcctgcaggagatggcaagaaaccctccattgcagctgtggtgggtagcatggatgctcaccccagcagatactgcgctaccgtccgtgtccagaggcccagacaggaggtgattcaggacctggcatccatggtgcgagagctgctcatccagttctacaagtctactcactacaaacccaccagaatcatcttctatagggatggagtgtctgaggggcagttcagacaggtgctgtattatgagctcctggccatacgtgaagcttgtatcagccttgagaaggaatatcagcctggaatcacctacatcgttgtgcagaaacgtcaccacactcgccttttctgtgctgaccgtgctgagagggttggccgcagtgggaacattccagcaggaaccacagtggacaccgacatcacccatccatacgaatttgacttttatctgtgcagtcatgctggaatacagggtacaagccgaccttcacactactacgtgctatgggacgacaactgcttcacagctgatgagttccagctcttgacgtaccagctgtgccacacatacgtgcgctgcactcgctccgtctccatccctgcaccagcctactatgctcatctggtggccttcagagcccgttatcatttagtggataaagagcatgacagtgcggaaggcagccatgtgtcgggtcagagtaacggtcgagaccctcaagccctggccaaagctgtgcagatccatcacgacactctgaggaccatgtactttgcctag

//