LOCUS       NM_001093519            3298 bp    mRNA    linear   VRT 27-APR-2025
DEFINITION  Xenopus laevis argonaute 2, RISC catalytic component L homeolog
            (ago2.L), mRNA.
ACCESSION   NM_001093519
VERSION     NM_001093519.1
KEYWORDS    RefSeq.
SOURCE      Xenopus laevis (African clawed frog)
  ORGANISM  Xenopus laevis
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Amphibia; Batrachia; Anura; Pipoidea; Pipidae; Xenopodinae;
            Xenopus; Xenopus.
REFERENCE   1  (bases 1 to 3298)
  AUTHORS   Klein,S.L., Strausberg,R.L., Wagner,L., Pontius,J., Clifton,S.W.
            and Richardson,P.
  TITLE     Genetic and genomic tools for Xenopus research: The NIH Xenopus
            initiative
  JOURNAL   Dev Dyn 225 (4), 384-391 (2002)
   PUBMED   12454917
COMMENT     PROVISIONAL REFSEQ: This record has not yet been subject to final
            NCBI review. The reference sequence was derived from BC077863.1.
            
            ##Evidence-Data-START##
            Transcript exon combination :: BC077863.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMD00012418, SAMD00012419
                                           [ECO:0000348]
            ##Evidence-Data-END##
FEATURES             Location/Qualifiers
     source          1..3298
                     /organism="Xenopus laevis"
                     /mol_type="mRNA"
                     /db_xref="taxon:8355"
                     /chromosome="6L"
                     /map="6L"
     gene            1..3298
                     /gene="ago2.L"
                     /gene_synonym="ago2; ago2.S; Argonaute2; eif2c1; eif2c2"
                     /note="argonaute 2, RISC catalytic component L homeolog"
                     /db_xref="GeneID:446823"
                     /db_xref="Xenbase:XB-GENE-1001922"
     misc_feature    140..142
                     /gene="ago2.L"
                     /gene_synonym="ago2; ago2.S; Argonaute2; eif2c1; eif2c2"
                     /note="upstream in-frame stop codon"
     CDS             263..2851
                     /gene="ago2.L"
                     /gene_synonym="ago2; ago2.S; Argonaute2; eif2c1; eif2c2"
                     /EC_number="3.1.26.n2"
                     /note="eukaryotic translation initiation factor 2C, 1;
                     argonaute 2, RISC catalytic component S homeolog;
                     argonaute RISC catalytic component 2"
                     /codon_start=1
                     /product="protein argonaute-2"
                     /protein_id="NP_001086988.1"
                     /db_xref="GeneID:446823"
                     /db_xref="Xenbase:XB-GENE-1001922"
                     /translation="

MYSGAGPVLVPPTTTPPLPMPAYTFKPPPRPDFGTSGRTIKLQANVFEMDIPKIEIYHYDIDIKPEKCPRRVNREIVEHMVQHFKAQIFGDRKPVFDGRKNLYTAMPLPIARDKQVELEVTLPGEGKDRIFKVAIKWMACVSLQALHDALSGRLPNVPFETVQALDVVMRHLPSMRYTPVGRSFFTASEGCANPLGGGREVWFGFHQSVRPSLWKMMLNIDVSATAFYKAQPVIEFMCEVLDFKSIEEQQKPLTDSQRVKFTKEIKGLKVEITHCGQMKRKYRVCNVTRRPASHQTFPLQQESGQTVECTVAQYFKDRHKLVLRYPHLPCLQVGQEQKHTYLPLEVCNIVAGQRCIKKLTDNQTSTMIRATARSAPDRQEEISKLMRSASFNTDPFVREFGIMVKDDMTDVTGRVLQPPSILYGGRSKAIATPVQGVWDMRNKQFHTGIEIKVWAIACFAPQRQCTEVHLKTFTEQLRKISRDAGMPIQGQPCFCKYAQGADSVEPMFRHLKNTYTGLQLVVVILPGKTPVYAEVKRVGDTVLGMATQCVQMKNVQRTTPQTLSNLCLKINVKLGGVNNILLPQGRPPVFQQPVIFLGADVTHPPAGDGKKPSIAAVVGSMDAHPNRYCATVRVQQHRQEIIQDLSAMVRELLIQFYKSTRFKPTRIIFYRDGVSEGQFQQVLHHELLAIREACIKLEKDYQPGITFIVVQKRHHTRLFCTDRNERVGKSGNIPAGTTVDTKITHPSEFDFYLCSHAGIQGTSRPSHYHVLWDDNRFSSDELQILTYQLCHTYVRCTRSVSIPAPAYYAHLVAFRARYHLVDKEHDSAEGSHTSGQSNGRDQQALAKAVQVHQDTLRTMYFA"

     misc_feature    509..769
                     /gene="ago2.L"
                     /gene_synonym="ago2; ago2.S; Argonaute2; eif2c1; eif2c2"
                     /note="N-terminal domain of argonaute; Region: ArgoN;
                     pfam16486"
                     /db_xref="CDD:465134"
     misc_feature    797..949
                     /gene="ago2.L"
                     /gene_synonym="ago2; ago2.S; Argonaute2; eif2c1; eif2c2"
                     /note="Argonaute linker 1 domain; Region: ArgoL1;
                     pfam08699"
                     /db_xref="CDD:462567"
     misc_feature    950..1312
                     /gene="ago2.L"
                     /gene_synonym="ago2; ago2.S; Argonaute2; eif2c1; eif2c2"
                     /note="PAZ domain, argonaute_like subfamily. Argonaute is
                     part of the RNA-induced silencing complex (RISC), and is
                     an endonuclease that plays a key role in the RNA
                     interference pathway. The PAZ domain has been named after
                     the proteins Piwi,Argonaut, and Zwille; Region:
                     PAZ_argonaute_like; cd02846"
                     /db_xref="CDD:239212"
     misc_feature    order(1106..1108,1151..1153,1193..1195,1205..1207,
                     1259..1261,1280..1282,1286..1288)
                     /gene="ago2.L"
                     /gene_synonym="ago2; ago2.S; Argonaute2; eif2c1; eif2c2"
                     /note="nucleic acid-binding interface [nucleotide
                     binding]; other site"
                     /db_xref="CDD:239212"
     misc_feature    1202..1219
                     /gene="ago2.L"
                     /gene_synonym="ago2; ago2.S; Argonaute2; eif2c1; eif2c2"
                     /note="propagated from UniProtKB/Swiss-Prot (Q6DCX2.1);
                     Region: Interaction with guide RNA. /evidence=ECO:0000250"
     misc_feature    1445..2722
                     /gene="ago2.L"
                     /gene_synonym="ago2; ago2.S; Argonaute2; eif2c1; eif2c2"
                     /note="PIWI domain, Argonaute-like subfamily. Argonaute is
                     the central component of the RNA-induced silencing complex
                     (RISC) and related complexes. The PIWI domain is the
                     C-terminal portion of Argonaute and consists of two
                     subdomains, one of which provides the...; Region:
                     Piwi_ago-like; cd04657"
                     /db_xref="CDD:240015"
     misc_feature    1841..1969
                     /gene="ago2.L"
                     /gene_synonym="ago2; ago2.S; Argonaute2; eif2c1; eif2c2"
                     /note="propagated from UniProtKB/Swiss-Prot (Q6DCX2.1);
                     Region: Interaction with guide RNA. /evidence=ECO:0000250"
     misc_feature    order(1856..1858,1868..1870,1904..1915,1922..1924,
                     1946..1948,1955..1957,1967..1969,1979..1981)
                     /gene="ago2.L"
                     /gene_synonym="ago2; ago2.S; Argonaute2; eif2c1; eif2c2"
                     /note="5' RNA guide strand anchoring site [active]"
                     /db_xref="CDD:240015"
     misc_feature    2030..2041
                     /gene="ago2.L"
                     /gene_synonym="ago2; ago2.S; Argonaute2; eif2c1; eif2c2"
                     /note="propagated from UniProtKB/Swiss-Prot (Q6DCX2.1);
                     Region: Interaction with GW182 family members.
                     /evidence=ECO:0000255"
     misc_feature    order(2060..2062,2066..2068,2276..2278,2690..2692)
                     /gene="ago2.L"
                     /gene_synonym="ago2; ago2.S; Argonaute2; eif2c1; eif2c2"
                     /note="active site"
                     /db_xref="CDD:240015"
     misc_feature    2219..2251
                     /gene="ago2.L"
                     /gene_synonym="ago2; ago2.S; Argonaute2; eif2c1; eif2c2"
                     /note="propagated from UniProtKB/Swiss-Prot (Q6DCX2.1);
                     Region: Interaction with GW182 family members.
                     /evidence=ECO:0000255"
     misc_feature    2396..2401
                     /gene="ago2.L"
                     /gene_synonym="ago2; ago2.S; Argonaute2; eif2c1; eif2c2"
                     /note="propagated from UniProtKB/Swiss-Prot (Q6DCX2.1);
                     Region: Interaction with guide RNA. /evidence=ECO:0000250"
     misc_feature    2528..2554
                     /gene="ago2.L"
                     /gene_synonym="ago2; ago2.S; Argonaute2; eif2c1; eif2c2"
                     /note="propagated from UniProtKB/Swiss-Prot (Q6DCX2.1);
                     Region: Interaction with guide RNA. /evidence=ECO:0000250"
     misc_feature    2639..2707
                     /gene="ago2.L"
                     /gene_synonym="ago2; ago2.S; Argonaute2; eif2c1; eif2c2"
                     /note="propagated from UniProtKB/Swiss-Prot (Q6DCX2.1);
                     Region: Interaction with guide RNA. /evidence=ECO:0000250"
     misc_feature    2735..2803
                     /gene="ago2.L"
                     /gene_synonym="ago2; ago2.S; Argonaute2; eif2c1; eif2c2"
                     /note="propagated from UniProtKB/Swiss-Prot (Q6DCX2.1);
                     Region: Disordered. /evidence=ECO:0000256|SAM:MobiDB-lite"
ORIGIN      

cggacgcgtgggcgttttcccctcagttcatgtcgcgcaaaagaaagaggaaaaaaaaaaagggaaagcagctagagaagcgagaggcgggaactcggcgtattccctcccacacaccgtgccttacaccatggagccataacggactgactggccgtctctgagggcttctgtgcggagcgaggggggcgggactggctacgttaccccctgtgtctggaactttattctcccctcacacttcgctgttgaagccgcagacatgtactccggggccgggccggtgcttgttccaccaaccacaacaccacctctcccaatgccggcatataccttcaagccacctccacggccagattttggtacctcagggaggaccatcaagctccaggcaaacgtttttgaaatggacattcccaaaattgaaatatatcactatgacattgatataaaaccagagaaatgccccagacgagtaaacagggaaatagtggaacacatggtccagcactttaaagctcaaatctttggggatcgaaaaccagtgtttgatggaagaaaaaacctttatactgcaatgcctctgccaattgcaagagataaacaggtggagttagaagtcacactacccggcgaagggaaagatcgtatcttcaaagttgcaatcaagtggatggcatgtgtaagcttgcaagccctgcacgacgctctgtctggtcgtcttcccaatgtcccttttgagacagttcaggctttagacgttgtgatgagacatttaccttctatgaggtatacacctgtcggtcgttcgttttttactgcatctgaaggctgcgcaaatcctcttgggggtggtagagaagtttggtttggcttccatcagtctgtacgaccttccctctggaaaatgatgctcaatatcgatgtgtctgcgacagcattttacaaagcacaaccagtaattgaatttatgtgtgaagttctggattttaaaagcatcgaagagcaacagaaacctcttaccgattcccaaagggttaagtttaccaaagaaattaaaggtttaaaagtcgaaataactcactgtggacaaatgaaacggaaataccgagtttgcaatgtaacaaggcgaccagccagtcatcaaacattccctcttcagcaggagagtggacagacggtcgagtgtaccgtcgcacagtacttcaaggacagacacaaactagttctgcgctaccctcatttaccatgtttacaggttgggcaggagcagaaacacacgtacctcccccttgaggtgtgcaatatagtggcaggccaaaggtgcataaagaaacttacggacaatcagacctccactatgatacgggcaactgctcgatctgctccagatcgccaggaagagatcagtaaattaatgcgaagtgcaagttttaatactgatccctttgtgcgagagtttggtataatggtgaaagatgacatgaccgatgtcacaggcagagtccttcaacctccttcaatcctttatggaggcaggagtaaagcaattgccacgcctgttcagggtgtctgggatatgagaaacaaacagttccacaccggcattgaaataaaggtgtgggccatcgcctgctttgccccacaacgacagtgtactgaagttcacctgaagacatttacagaacagttgaggaagatttctcgtgatgccgggatgcccatccaggggcagccatgtttctgcaagtatgcccagggagcagatagtgttgagccaatgttcaggcatctgaagaatacatatacagggctacaacttgttgttgtaatactgcctgggaaaacccctgtctatgcggaagtgaaacgtgtgggggacactgttctgggaatggctactcagtgtgttcagatgaaaaatgtgcagaggaccacaccacaaaccttgtctaacctgtgcttaaaaataaatgtaaaattaggaggcgtcaataacattttgttgccccagggcagacctcctgtgtttcaacagccagtcatatttcttggagctgatgttacccacccacctgcaggggacggtaaaaagccttcgattgctgcggttgtaggtagtatggatgcacaccccaaccgatactgtgccactgtgcgggtgcagcagcaccgacaagaaatcattcaggatttgtctgccatggttcgggagctgcttatccagttctacaagtctactcgcttcaagcccacaagaatcatcttttacagagatggtgtctcagaagggcagttccaacaggttctccatcatgagcttctggccattagggaagcctgtattaaactggagaaggattaccagccaggaattacatttattgtagtgcagaaaagacatcacacgcggctattctgtacagaccgaaatgagagggtagggaaaagtggaaatataccagctggtaccactgtagacacaaagattacccacccttcagagtttgatttttacctgtgcagtcacgctggtattcagggaacaagcaggccgtcacactatcatgtcctgtgggatgacaatcgcttttcatctgatgagttgcagatcctcacttatcagctctgccacacttatgttcgttgcactcgttctgtgtctatccctgctccagcatattatgctcacctggtggcattcagagcaaggtatcatttggtggataaagagcatgacagcgctgaaggtagccatacatctggtcagagtaacgggagagatcagcaagcactcgctaaggcagtccaggtgcatcaggacacactgcgtacaatgtactttgcataacagccgctgtttatccagtgacttaatagcactcctggaaaaggattcacacagaccagctgcacttagttgtttgtaaactagtccatactacgctgactcccatcaaaaagtgccggtaaccctgttgtctcccttactgtccaaccagccttccgtcccaaccgcttatcacattttgtttccattcacaactcagacctgtcacaattaatgtagcttggagtgtgattcgtatcggaatgtttacaaaggtgcagagagaaaaacacacatacccatgtagaaaaactgaacacgtaacctcagggctttagactttcactatggaatattgtttcagtttctgttttatccctgttcagttgctgaatttaatgggatggttgcggtcagttgggttggttacaaaaagagcataaaatgagcttaaaaaaaaaaaaaaaa

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